http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106754606-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ffb184bcc33359ad2a84a8499ef865fe |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-64 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2017-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01c07985f0ed630bea0351baf76f3755 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_401344dafd9ccd2e02b30c48c7694316 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12b74c7d3f7f741a8e361852878ec14a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e16ab79568e8a9aeceb25106ae865558 |
| publicationDate | 2017-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106754606-A |
| titleOfInvention | Screening method for markerless deletion mutants of X. citri hfq gene |
| abstract | The invention discloses a screening method for the unmarked deletion mutant of X. citri hfq gene, which comprises the following steps: (1) extracting the DNA of X. citri; (2) constructing a recombinant suicide plasmid vector with hfq gene deleted: A. ‑up‑F, Hfq‑up‑R, Hfq‑down‑F, Hfq‑down‑R respectively amplify the upper and lower flanking sequences of the hfq gene of X. citri; B, recover the PCR fragment; C, mix the recovered product with the carrier respectively pEASY‑T 1 vector ligation; D, the ligated products were transformed into Escherichia coli respectively; E, the positive cloned plasmid was extracted; F, the double enzyme digestion upstream/downstream T‑vector recombination plasmid, pK18mobSacB; DH5α Escherichia coli; (3) transforming the recombinant suicide plasmid vector into X. citri; (4) screening mutants by sucrose medium method. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108753675-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116042688-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116042688-B |
| priorityDate | 2017-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.