| abstract |
The invention discloses recombinant bacillus subtilis for high yield of heparosan and application thereof, belonging to the technical field of biological engineering. In the invention, key enzyme genes in a UDP-GlcNUA pathway and a UDP-GlcNAc pathway are co-expressed in the Bacillus subtilis for producing heparosan, and a recombinant strain with increased yield and molecular weight of the heparosan is obtained. When the tuaD, gtaB, glmU, glmM and glmS genes were co-expressed, the yield of heparosan in shake flasks increased to 2.89g/L with a molecular weight of 75.90 kDa. By fed-batch fermentation in a 3L fermenter, the yield of heparosan finally accumulates to 7.25g/L with a molecular weight of 46.66 kDa. The invention lays a foundation for the industrial production of heparin. |