http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106754477-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M23-58 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-10 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 |
filingDate | 2016-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106754477-B |
titleOfInvention | Continuous culture method of bacillus licheniformis and special fermentation system |
abstract | The invention belongs to the field of microorganisms, and particularly relates to a continuous culture method and a special fermentation system for bacillus licheniformis. The method comprises the following steps: (1) transferring the bacillus licheniformis strain to a test tube slant culture medium, activating the strain, and sequentially transferring the strain to a triangular flask and a seeding tank for culture to prepare bacillus licheniformis seeds; (2) taking a culture material: starch/glucose, corn flour, soybean meal, (NH) 4 ) 2 HPO 4 、NaCl、CaCl 2 、MgSO 4 .7H 2 O、KH 2 PO 4 、K 2 HPO 4 、MnSO 4 Defoaming agent and water; (3) and (3) sterilizing the compost obtained in the step (2), inoculating activated and cultured bacillus licheniformis seeds, and performing three-stage continuous culture to obtain the final fermentation liquor. The method of the invention realizes high mechanization and automation, the quality of the bacillus licheniformis is more stable and uniform, and the mixed bacteria pollution and the strain variation are not easy to occur; the service life of each detection instrument is prolonged; time is saved, and yield and production efficiency are improved; improves the concentration and the spore rate of the bacillus licheniformis. |
priorityDate | 2016-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.