http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106754444-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-34 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-721 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6897 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2016-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106754444-B |
| titleOfInvention | For detecting the recombination yeast and its construction method of estrogen compound |
| abstract | The invention discloses the recombination yeasts and its construction method for detecting estrogen compound.A kind of recombinant bacterium provided by the invention, to express Estrogen receptor β gene, resistance screening expression casette, 3 series connection ERE genes and reporter gene expression box, obtained recombinant bacterium in saccharomyces cerevisiae.Using recombination yeast constructed by the method for the present invention in the presence of estrogens compounds, can stability and high efficiency synthesize GFP, the expression quantity of GFP and the concentration of estrogens compounds are proportional, and the EC50 of 17 β estradiol is 3.25pM, improve about 20 times than existing literature report.The present invention is to rapidly and sensitively detect estrogens compounds in environment to lay the foundation. |
| priorityDate | 2016-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.