http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106749623-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-765 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-765 |
| filingDate | 2016-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106749623-B |
| titleOfInvention | Method for separating human albumin by expanded bed adsorption based on mixed mode |
| abstract | The invention discloses a method for separating human serum albumin by expanded bed adsorption based on a mixed mode, which can directly separate recombinant human serum albumin from yeast fermentation liquor and belongs to a protein separation technology in the field of biochemical engineering. The method comprises the following specific steps: 1) pretreating fermentation liquor, namely adding sodium caprylate into yeast fermentation liquor containing human serum albumin, and heating to inactivate protease; 2) expanded bed adsorption, directly separating yeast fermentation liquor by using the expanded bed filled with mixed mode adsorbent, and collecting elution peak. 3) Desalting and drying, desalting the collected liquid, and freeze-drying to obtain human serum albumin with the purity of more than 95%. The invention is characterized in that a new separation process is developed, and high-purity recombinant human serum albumin can be directly separated and obtained from yeast fermentation liquor. The key point of the method is that the expanded bed adsorbent based on the mixed mode is adopted, the yeast fermentation liquid does not need to remove cells, does not need to adjust the ionic strength, and has the characteristics of mild elution condition, simple process, high separation efficiency and high yield. |
| priorityDate | 2016-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.