http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106749562-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2469-20 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56911 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
filingDate | 2016-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106749562-B |
titleOfInvention | Gene expression product BLSJ-1 with brucella diagnosis and identification functions and preparation method thereof |
abstract | The invention relates to a gene expression product BLSJ-1 with brucella diagnosis and identification functions and a preparation method thereof. The product is obtained by prokaryotic expression of a Brucella S2 strain gene with the gene number (GI) of 489054867 and purification through a Glutathione S Transferase (GST) affinity chromatographic column and a glutathione gradient elution method. The product is used as a coating antigen and can be used as a diagnostic antigen for distinguishing animal brucella vaccine immunity and natural infection serum detection. The antigen is respectively carried out immunoblotting (Western-blot) with vaccine immune antibody and natural infection antibody, and the difference is obvious. The BLSJ-1 antigen is used as an indirect enzyme-linked immunosorbent assay (ELISA) envelope antigen to detect hundreds of clinical serum samples with the disease, and the differential diagnosis effect is obvious. |
priorityDate | 2016-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.