http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106732474-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-62 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 |
filingDate | 2017-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106732474-B |
titleOfInvention | Magnetic nano material, preparation method thereof and application of magnetic nano material in enrichment analysis of glycosylated peptide segments |
abstract | The invention belongs to the technical field of biotechnology and nano materials, and particularly relates to a cysteamine hydrochloride modified ferroferric oxide magnetic nano material, a preparation method thereof and application thereof in enrichment analysis of glycosylated peptide segments. Mixing a ferroferric oxide magnetic nano material modified by cysteamine hydrochloride and a glycosylated peptide section solution in an acetonitrile/water/trifluoroacetic acid mixed solution, and incubating in an enzymolysis instrument; separating the nanometer material under the action of an external magnetic field to remove supernatant, and washing the nanometer material by using acetonitrile/water/trifluoroacetic acid mixed solution; and eluting the glycosylation peptide sections enriched on the material by using acetonitrile/water/formic acid mixed solution, and combining mass spectrometry analysis and identification. The method has the advantages of simple and rapid operation, recyclable materials, low cost, high sensitivity and good selectivity, is very suitable for enrichment analysis of glycosylated peptide segments, and has good application prospect. |
priorityDate | 2017-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.