http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106701774-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-102 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43522 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 |
| filingDate | 2016-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106701774-B |
| titleOfInvention | Preparation method of recombinant scorpion neurotoxin LqhIT2 protein |
| abstract | The invention discloses a method for efficiently preparing recombinant active scorpion and insect neurotoxin LqhIT2, which mainly comprises the following steps: (1) optimizing and synthesizing LqhIT2 gene with 6 × His label at C-end according to the amino acid sequence of LqhIT2 mature toxin and the characteristics of a pichia pastoris expression system, and constructing a pichia pastoris secretion expression vector; (2) transforming the recombinant vector into a pichia host, and screening to obtain a yeast transformant with high-level secretory expression; (3) the recombinant scorpion insect neurotoxin LqhIT2 protein is expressed in a yeast host, and the recombinant scorpion insect neurotoxin LqhIT2 protein with the purity of more than 98 percent is quickly obtained through a nickel affinity chromatography method, and the purified recombinant protein has strong poisoning activity on insect cells. The invention changes the traditional expression of insect neurotoxin LqhIT2 by prokaryotic host escherichia coli, explores a method for expressing insect neurotoxin LqhIT2 by eukaryotic host pichia pastoris, and screens yeast transformants with high-level secretory expression. |
| priorityDate | 2016-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.