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filingDate 2017-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2017-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106701766-A
titleOfInvention Internal reference plasmid suitable for silkworm cell double luciferase reporter gene system and preparation method and application of internal reference plasmid
abstract The invention relates to an internal reference plasmid suitable for a silkworm cell double luciferase reporter gene system and a preparation method and application of the internal reference plasmid. The internal reference plasmid contains a constitutive promoter BmVgP78M suitable for the silkworm cell double luciferase reporter gene system, wherein the promoter is obtained by a BmVg gene promoter mutant hormone element to constitute the internal reference plasmid which does not respond to a silkworm hormone, can be stably expressed in silkworm cells, has moderate activity, and can be effectively used for the silkworm cell double luciferase reporter gene system to study the promoter activity or gene regulation.
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