http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106701678-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a878a0e76a615985995108f9ae00e2a1 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96db2d2ab8202f3659684d6a232578ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3357b6fd9e2393cc0cf9b0f1bfc186c4 |
| publicationDate | 2017-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106701678-A |
| titleOfInvention | Method for efficient preparation of CIK cell |
| abstract | The invention relates to the field of biotechnology, in particular to a method for efficient preparation of a CIK cell. The method is characterized by adopting the following preparation steps: preliminary separation of lymphocytes; twice washing with lotion; lymphocyte concentration regulation; culture: adding the concentration regulated lymphocytes into a cell bottle coated with CD3 antibody to conduct culture for 24h, then adding 1ml of an IFN-gamma reagent and 1ml of an IL-1beta reagent to perform culture for 48h to obtain the CIK cell; enlarged culture; and collection. Compared with the prior art, the method provided by the invention adopts coating with the CD3 antibody firstly, then adds IFN-gamma, also breaks the routine and uses IL-1beta, through coating, the cell can contact the antibody more lastingly, and a better activation effect can be achieved with a small dosage. The CIK cell prepared by the method provided by the invention has cytotoxic activity obviously higher than that of existing CIK cells by more than 30%, the problems of poor reproductive capacity and low cell viability of CIK cells are solved, and the cost is greatly lowered. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111117959-A |
| priorityDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 78.