http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106676134-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0651 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
filingDate | 2016-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106676134-B |
titleOfInvention | Method for obtaining type II intrinsic lymphocytes from mice and carrier used in method |
abstract | The invention provides a method for obtaining type II inherent lymphocyte from a mouse body and a carrier used by the method, wherein the carrier is a recombinant expression carrier obtained by connecting a coding sequence of kappa chain signal peptide of mouse IgG and a coding sequence of murine IL-33 active region to a pcDNA3.1 eukaryotic expression carrier through a gene recombination technology. The recombinant expression vector is introduced into an animal body, so that murine IL-33 is overexpressed in the animal body, and the II type inherent lymphocyte is induced and amplified in the liver; then separating to obtain liver tissue lymphocyte, marking the obtained liver tissue lymphocyte with type II inherent lymphocyte specific antibody, and sorting by cell flow technology to obtain type II inherent lymphocyte. The technology of the invention can induce and largely amplify, separate and purify high-activity type II inherent lymphocytes from C57BL/6 mice. |
priorityDate | 2016-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 144.