http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106676132-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8222 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2017-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106676132-B |
| titleOfInvention | Efficient plant binary induction gene expression recombinant plasmid |
| abstract | A high-efficiency plant binary induction gene expression recombinant plasmid is realized by the following steps: constructing a DR5 promoter induced by auxin IAA, and promoting the BD domain expression of a yeast transcriptional activator protein Gal4 by the promoter; constructing a promoter induced by low temperature, and using the promoter to start the AD structural domain expression of the yeast transcriptional activator protein Gal 4; constructing a promoter activated by Gal4 to start the expression of a reporter gene GFP; and (3) introducing the three expression segments into the same plant expression vector pBI 121. The invention can induce the expression of the reporter gene only when two inducing conditions exist simultaneously, thus reducing the interference; meanwhile, the invention realizes the two-stage amplification of the induction signal by utilizing the basic principle of the Gal4/UAS system, and can improve the detection sensitivity of the induction signal; in addition, the invention also has great flexibility and extensibility, and various DNA elements responding to chemical signals and physical signals can be used for constructing a binary inducible expression system and can be widely applied to the research of modern genetics and molecular biology. |
| priorityDate | 2017-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 180.