http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106674134-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-182 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D239-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K11-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D239-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2016-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106674134-B |
| titleOfInvention | A kind of novel fluorescence probe and preparation method thereof and the application in detection 6-thioguanine |
| abstract | The invention discloses a kind of novel fluorescence probes and preparation method thereof and the application in detection 6-thioguanine.Dtpa, acetic anhydride are taken, pyridine is slowly stirred heating for 24 hours at 65 DEG C.It is cooled to room temperature, filters, anhydrous ether washing filters, and vacuum drying obtains dtpaa.Dtpaa is taken, triethylamine, DMF, cytimidine, 100 DEG C of heating stirrings of constant temperature are for 24 hours.It is cooled to room temperature, is filtered, vacuum drying obtains dtpa-bis (cytosine).It takes dtpa-bis (cytosine) to be dissolved in the Tris-HCl buffer solution of pH=7.4, and washs Eu (NO with this solution 3 ) 3 ยท6H 2 O, collects cleaning solution, and heating or for a long time placement obtain fluorescence probe.Fluorescence probe combination fluorescent method of the invention detects 6-TG, and method is simple and novel, high-efficient and at low cost, not will cause environmental pollution. |
| priorityDate | 2016-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.