http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106661631-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2015-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106661631-B |
| titleOfInvention | Methods for specifically targeted capture of regions of the human genome and transcriptome from blood |
| abstract | The present invention relates to a method for highly specific targeted capture of regions of the human genome and transcriptome from blood (ie, from cell-free circulating DNA, exosomes, microRNA, circulating tumor cells, or total blood cells) to allow for Highly sensitive detection of mutations, expression, copy number, translocations, alternative splicing and methylation changes using combined nuclease, ligation, polymerase and massively parallel sequencing reactions. The method produces a collection of distinct circular chimeric single-stranded nucleic acid constructs suitable for sequencing on multiple platforms. In some embodiments, each construct of the collection comprises a first single-stranded segment of original genomic DNA from a host organism and linked to the first single-stranded segment and comprising A second single-stranded synthetic nucleic acid segment referred to as an exogenous nucleotide sequence. These chimeric constructs are suitable for identifying and enumerating mutations, copy changes, translocations and methylation changes. In other embodiments, the input mRNA, IncRNA, or miRNA is used to generate a circular DNA product that reflects the presence and copy number of specific mRNAs, lncRNAs, splice site variants, translocations, and miRNAs. |
| priorityDate | 2014-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 341.