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classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-37
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
filingDate 2016-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2018-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2018-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106645686-B
titleOfInvention One kind being directed to fumonisin B1Sensitive detection method
abstract The present invention provides one kind being directed to fumonisin B 1 Sensitive detection method, this method substitutes conventional zymophore and fumonisin B using the fluorescent microsphere for being embedded with quantum dot 1 Coupling executes direct competive ELISA using coupled product as competition antigen.In technology path, the present invention is first depending on the characteristics of luminescence and has selected suitable quantum dot, further devises the quantum dot embedding method based on fluorescent microsphere technology, on this basis, by quantum dot fluorescence microballoon after BSA is coated with fumonisin B 1 Coupling, to obtain performance preferably competitive antigen.In the technical solution, fluorescent microsphere has embedded a large amount of quantum dot by polymer support, thus has higher luminous intensity, can effectively improve the sensitivity of detection;Moreover, because fluorescent microsphere has larger grain size, therefore can reduce affinity excessively high between competition antigen and coated antibody to a certain extent, to promote the sensitivity of detection.
priorityDate 2016-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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