http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106645071-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a9e6a0102f7dc0397aaeb636aa13eafb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2017-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30890971b1ab6a87d1b3b4670e89936c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71d4c4c6126720d34f761455320b2087 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a8485a435bcf367a9fcb99e808229f2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f350970a460c01927b7892dbddb1b2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_968f785fca959a20e74214bd5ad34567 |
| publicationDate | 2017-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106645071-A |
| titleOfInvention | Method for Determination of Urease Based on Nano-copper Oxide |
| abstract | The invention discloses a method for measuring urease based on nano-copper oxide, which simulates the activity of alkaline peroxidase based on nano-copper oxide, combined with urease, can selectively hydrolyze urea to generate ammonia and carbon dioxide, and inhibits nano-copper oxide from ammonia to catalyze the oxidation of hydrogen peroxide The process of p-hydroxyphenylpropionic acid reduces the generation of dimer fluorescent products, and provides a fluorescence analysis method for the determination of urease using nano-copper oxide as a catalyst. It is to mix urea, urease and phosphate buffer in a warm bath for the enzymatic reaction of urease, and then add p-hydroxyphenylpropionic acid, hydrogen peroxide, nano-copper oxide and phosphate buffer to continue the warm bath for fluorescence reaction. The maximum excitation wavelength and emission wavelength of the reaction product are 320 nm and 409 nm, respectively. The linear range of urease was 0.003~0.04 U/mL, and the detection limit was 2.6 U/L. This method can be used for the determination of urease in soil. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112044471-A |
| priorityDate | 2017-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.