http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106636205-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23V2002-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-168 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L33-185 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-10 |
filingDate | 2017-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106636205-B |
titleOfInvention | A method for expressing polypeptide PA1B |
abstract | The invention provides a method for efficiently expressing polypeptide PA1b, comprising the following steps: (1) synthesis of target gene PA1b; (2) connection of PA1b gene and carrier; (3) transformation of recombinant plasmid PUSCN1-PA1b; (4) Recombinant plasmid digestion and sequencing identification; (5) 293F (human embryonic kidney suspension cell) cell transfection; the main inventive point of the present invention is that human-derived mammalian cells are used to successfully express PA1b for the first time, the expressed protein is stable in quality, and the product It is uniform and has a purity of 98%, and the polypeptide PA1b protein of the present invention can be used in the manufacture of medicines and foods for the treatment of diabetes. Its manufacturing cost is lower than that of insulin, and it is more convenient than insulin. Below the physiological level, it does not exert a hypoglycemic effect. |
priorityDate | 2017-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 127.