http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106636204-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-106 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y110-03001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0059 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
| filingDate | 2017-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106636204-B |
| titleOfInvention | A kind of albefaction Misgurnus auguillicaudatus breeding method that can stablize heredity |
| abstract | The invention discloses the albefaction Misgurnus auguillicaudatus breeding methods that one kind can stablize heredity, it is the design TYR gene target site in Misgurnus auguillicaudatus TYR gene order, then upstream primer and matched downstream primer are designed, PCR amplification is carried out by template of skeleton plasmid, is transcribed in vitro, obtains gRNA after purification；To linearize Cas9 plasmid as template, it is transcribed in vitro, obtains Cas9mRNA after purification；To Misgurnus auguillicaudatus fertilized eggs microinjection, fertilized eggs are subjected to hatching, cultivating later, and hybridize to gene knockout fish, obtain the albefaction Misgurnus auguillicaudatus that can stablize heredity. |
| priorityDate | 2017-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 100.