http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106636173-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-34 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-18 |
| filingDate | 2017-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106636173-B |
| titleOfInvention | Enhanced Green Fluorescent Protein Marking Method for Maize Head Smut Strain and Its Strain and Application |
| abstract | The invention relates to the technical field of plant pathology, in particular to a method for marking corn head smut strains with enhanced green fluorescent protein, as well as strains and applications thereof. The method provided by the present invention for marking corn head smut strains with enhanced green fluorescent protein constructs a carrier PHD3-mig2-5-1-EGFP that uses a protoplast genetic transformation method to regulate the expression of EGFP by a mig2-5-1 promoter. The vector is transferred into the head smut of corn, and the mig2-5-1 promoter can be used to induce EGFP to obtain a large amount of highly efficient fluorescent expression in the strain, and at the same time, it can be genetically expressed stably by PCR method and fluorescence test. The enhanced green fluorescent protein-labeled corn head smut strain provided by the present invention can be used in the screening and identification of corn varieties resistant to corn head smut, the identification result is reliable, the operation is convenient, simple and easy, the identification period is short, and no Affected by the environment, the repeatability is high. |
| priorityDate | 2017-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.