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filingDate 2016-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106636168-B
titleOfInvention Method for regulating and controlling synthesis of clostridium acetobutylicum extracellular polymer
abstract The invention discloses a method for regulating and controlling synthesis of an extracellular polymer of clostridium acetobutylicum, which is characterized in that an intron sequence is inserted into a Spo0A gene of clostridium acetobutylicum by using a two-type intron gene knockout technology. The invention provides a simple and efficient method for regulating and controlling EPS synthesis, the recombinant strain obtained by the method has higher regulation and control effect on EPS, when glucose is used as a carbon source and a 2. gamma. bacterium biofilm is cultured in a 100ml anaerobic bottle by a glass slide biofilm culture method, the biofilm thickness is 5.81 mu m, and the biofilm thickness of a starting strain cultured under the same condition reaches 24.6 mu m.
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