Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-145 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-145 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-26 |
filingDate |
2016-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2020-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate |
2020-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-106636168-B |
titleOfInvention |
Method for regulating and controlling synthesis of clostridium acetobutylicum extracellular polymer |
abstract |
The invention discloses a method for regulating and controlling synthesis of an extracellular polymer of clostridium acetobutylicum, which is characterized in that an intron sequence is inserted into a Spo0A gene of clostridium acetobutylicum by using a two-type intron gene knockout technology. The invention provides a simple and efficient method for regulating and controlling EPS synthesis, the recombinant strain obtained by the method has higher regulation and control effect on EPS, when glucose is used as a carbon source and a 2. gamma. bacterium biofilm is cultured in a 100ml anaerobic bottle by a glass slide biofilm culture method, the biofilm thickness is 5.81 mu m, and the biofilm thickness of a starting strain cultured under the same condition reaches 24.6 mu m. |
priorityDate |
2016-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |