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filingDate 2016-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106636152-B
titleOfInvention A kind of gene, expression vector, bacterial strain and its application of paper grade (stock) sterol lipase
abstract The present invention discloses gene, expression vector, bacterial strain and its application of a kind of paper grade (stock) sterol lipase, belongs to bioengineering field.The nucleotide coding sequence of the gene is as shown in SEQ ID NO:1.Sterol lipase gene provided by the invention realizes the high expression in Pichia pastoris using technologies such as codon optimization, Bacillus coli expression, Pichia anomala expressions;Obtained sterol lipase enzyme activity is 0.4U/mL or so.The sterol lipase of expression has high temperature resistant, resistance to weak base property;Sterol lipase of the present invention can be applied to deinking, remove ink particle by the gluing object between hydrolysis waste paper surface and paper, it is ensured that the stabilization of recycled paper quality and the operational efficiency of paper machine.
priorityDate 2016-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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