http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106636019-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C33-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07B2200-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-2207 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01184 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C29-143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-641 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2016-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106636019-B |
| titleOfInvention | Sortase A-mediated (S)-carbonyl reductase oligomers catalyze the synthesis of (S)-phenylethylene glycol efficiently |
| abstract | The invention discloses the synthesis of (S)-carbonyl reductase oligomer mediated by Sortase A with high efficiency for catalyzing (S)-phenylethylene glycol, and belongs to the field of biocatalytic asymmetric transformation. In the present invention, the (S)-carbonyl reductase introduced into the SrtA recognition sequence is purified, and the pure SrtA enzyme is obtained by purification at the same time, and the (S)-carbonyl reductase oligomer is obtained by using the SrtA-mediated ligation reaction; The polymer is the catalyst, 2-hydroxyacetophenone is the substrate, the reaction time is 3 hours, the optical purity of the product (S)-phenylethylene glycol reaches 100% e.e., and the yield is 99%; The enzymatic activity of the polymer was increased by 6 times, the time for catalytic conversion of (S)-phenylethylene glycol was shortened by 16 times, and the thermal stability was improved by 10 °C. The invention solves the defects of low catalytic efficiency and poor thermal stability of carbonyl reductase by using SrtA-mediated protein connection, provides an effective way for efficient preparation of (S)-phenylethylene glycol, and provides a new direction for the field of protein modification . |
| priorityDate | 2016-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 323.