http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106635995-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-599 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 |
| filingDate | 2017-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106635995-B |
| titleOfInvention | Negative enrichment method for circulating tumor cells |
| abstract | The invention relates to the field of molecular biology, in particular to a negative enrichment method of circulating tumor cells. The density gradient centrifugation based on the lymphocyte separation tube is adopted to carry out pretreatment on the blood sample, so that the operation steps of separating leucocytes are simplified, the enrichment time of circulating tumor cells is shortened, and the whole operation can be finished within 2.5 hours; the number of the left white blood cells is 30-50% of the original number of the white blood cells of the blood sample, the left white blood cells are marked with self-made negative enrichment immunomagnetic beads CD45 and are removed, the using amount of the immunomagnetic beads is reduced, and the cost of negative enrichment of circulating tumor cells is greatly saved; the invention adopts the lymphocyte separation tube to pretreat the blood sample, improves the negative enrichment method of the circulating tumor cells, effectively improves the stability of enrichment operation, and ensures that the recovery rate of the circulating tumor cells reaches more than 85 percent; the invention also has the advantage of high sensitivity, and can stably detect the circulating tumor cells in a sample with extremely small quantity. |
| priorityDate | 2017-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.