http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106635990-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0622 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 |
| filingDate | 2016-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106635990-B |
| titleOfInvention | Method for primary culture of dorsal root ganglion satellite glial cells |
| abstract | The invention discloses a method for primary culture of dorsal root ganglion satellite glial cells, which comprises the following steps: disinfecting a newborn mouse or a rat, cutting off the head, killing, taking out a spine after dissection, removing muscles on the spine, cutting off the spine, cleaning blood vessels and the spinal cord under a stereoscopic microscope, taking out a DRG, and removing nerve fibers and capsules on the DRG; culturing the treated DRG by adopting a DRG-SGCs culture solution, inducing a large amount of satellite glial cells to migrate out of the DRG, adding trypsin for digestion after the culture is finished, then adding FBS for terminating the digestion, slightly blowing by adopting a Pasteur suction pipe, and transferring to a centrifuge tube for centrifugation; and (4) centrifuging, removing the supernatant, adding a culture solution, and continuously culturing to obtain the satellite glial cells. The cultured and purified satellite glial cells can survive for a long time in vitro, have high purity and stably survive, and can be passaged for multiple generations to form a cell network. |
| priorityDate | 2016-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 130.