http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106613997-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2017-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106613997-B |
| titleOfInvention | A kind of tree peony Regeneration in Vitro tissue culture method |
| abstract | A kind of tree peony Regeneration in Vitro tissue culture method, takes its mature embryo after first Paeonia suffruticosa seed sterilizes, be seeded on 1/2MS culture mediums and cultivate；Its tender hypocotyl is taken after ripe embryo germination, is seeded in modified MS medium and is cultivated 15 25 days, until hypocotyl incision is expanded；The hypocotyl subculture that incision is expanded is inoculated with 14 30 days in modified MS medium again, until the incision that hypocotyl is expanded directly differentiates tree peony adventitious bud；When tree peony adventitious bud is grown to 3 4cm, tree peony adventitious bud is cut, and be seeded in modified MS medium and take root from root, that is, completes tree peony Regeneration in Vitro.A kind of tree peony Regeneration in Vitro tissue culture method provided by the invention, in the unexpected releasing of PEG6000 osmotic stresses and Ca 2+ With micro- Mn 2+ 、Zn 2+ 、Mo 6+ And Cu 2+ Concentration it is increased in the case of, hypocotyl can generate adventitious bud without callus and directly, skip over callus this process, solve the problems, such as that callus differentiation adventitious bud is relatively difficult, Differentiation ration of adventitious buds is general relatively low. |
| priorityDate | 2017-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.