http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106608908-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-39 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-39 |
filingDate | 2015-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106608908-B |
titleOfInvention | Method for extracting glucose tolerance factor from chromium-containing yeast |
abstract | The invention discloses a method for extracting a glucose tolerance factor from chromium-containing yeast. The method comprises the following steps: 1) extracting chromium-containing yeast powder with ammonia water to obtain an ammonia water extract; 2) and under the ice bath condition, extracting the ammonia water extracting solution by using an ethanol water solution, centrifuging, collecting supernate, and removing ethanol to realize the extraction of the glucose tolerance factor. The extraction method is based on the following principle: the compound of small molecular protein and chromium (GTF) can be dissolved in ammonia water and alcohol solution, while the inorganic chromium which does not produce biotransformation in thallus is not dissolved in alcohol solution or precipitated in ammonia water solution. Based on the characteristic of low GTF content in yeast, the invention adopts a method of combining ammonia water extraction and ethanol precipitation extraction, improves the GTF content in GTF extract, and reduces the content of impurity protein to a greater extent, thus being a method for effectively extracting GTF. |
priorityDate | 2015-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 111.