| abstract |
The invention discloses a chemiluminescent detection kit for a bovine foot-and-mouth disease 3ABC antibody and belongs to the field of immunological detection. The chemiluminescent detection kit comprises a 96-well chemiluminescent immunoassay plate, an enzyme-labeled antibody, a serum diluent, a chemiluminescent substrate, a chemiluminescent enhancer and PBST washing liquid, wherein the 96-well chemiluminescent immunoassay plate is coated with 3ABC fusion protein, the enzyme-labeled antibody is a horseradish peroxidase labeled rabbit anti-bovine lgG antibody, the serum diluent comprises 0.01% of Tween-20, 1% of casein, 10% of horse serum and 1% of escherichia coli, the chemiluminescent substrate refers to a Luminol solution, and the chemiluminescent enhancer comprises IPP, H2O2 and Tween-20. Compared with commercially-available kit products, the chemiluminescent detection kit is high in sensitivity, higher in specificity and superior to other products in diagnosibility. |