| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0d6a852e9b5a15aba6718e9b663ccd9b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-002 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
| filingDate |
2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fb790f81b1c79a7eb1a423725547423 |
| publicationDate |
2017-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-106591258-A |
| titleOfInvention |
A lipase gene, carrier, engineering bacteria and application thereof |
| abstract |
The invention belongs to the field of genetic engineering and discloses a lipase whose amino acid sequence is shown in SEQ ID NO:2. Link its coding gene with the expression vector to construct a recombinant plasmid for intracellular expression containing the gene, and then transform it into an E. coli strain to obtain recombinant E. coli, which can be used as a catalyst to biocatalyze the production of pregabalin. Preparation of (3S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid from chiral intermediates. The lipase obtained by the invention has extremely high enantioselectivity for ethyl 2-carboxyethyl-3-cyano-5-methylhexanoate, and has good industrial application prospects. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023032952-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112301017-A |
| priorityDate |
2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |