http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106568949-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-588 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54306 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate | 2016-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106568949-B |
| titleOfInvention | A kind of small haptens detection method based on direct competitive fluoroimmunoassay |
| abstract | The present invention provides a kind of small haptens detection methods based on direct competitive fluoroimmunoassay, this method substitutes conventional zymophore using the fluorescent microsphere for being embedded with quantum dot and is coupled with small haptens, and direct competive ELISA is executed using coupled product as competition antigen.In the technical solution, fluorescent microsphere has embedded a large amount of quantum dot by polymer support, thus has higher luminous intensity, can effectively improve the sensitivity of detection.Further, since quantum dot is wrapped in the inside of microballoon, be affected by the external environment small, avoid to a certain extent fluorescence be quenched and the coagulation of microballoon.Simultaneously as quantum dot microsphere has relatively large grain size, therefore simplicity is isolated and purified compared with quantum dot, the slow-speed of revolution (<10000rpm) centrifugation can be achieved with the separation of the microballoon in conventional soln；Moreover, larger grain size can reduce affinity excessively high between competition antigen and coated antibody to a certain extent, to promote the sensitivity of detection. |
| priorityDate | 2016-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.