http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106566890-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 |
filingDate | 2016-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106566890-B |
titleOfInvention | Method for developing rape microsatellite marker locus and method for detecting length of microsatellite marker in microsatellite marker locus |
abstract | The invention discloses a development method of rape microsatellite marker loci and a length detection method of microsatellite markers in the microsatellite marker loci. The development method comprises the following steps: obtaining a mixed sample; extracting the genome of the mixed sample; fragmenting a genome to obtain a genome fragment; respectively hybridizing the probe sets with the genome fragments; purifying the successfully hybridized genome fragments in the plurality of hybridization solutions; mixing a plurality of the purified hybrid genomic fragments, and detecting the purified genomic fragments by using high-throughput sequencing; obtaining an effective said high throughput sequencing fragment; classifying the valid high throughput sequencing fragments. The detection method comprises the following steps: selecting a microsatellite marker locus to be detected; and amplifying the microsatellite marker in the microsatellite marker locus to be detected by using a multiplex amplification primer to obtain the length of the microsatellite marker in the microsatellite marker locus. The method is simple, rapid, comprehensive and accurate. |
priorityDate | 2016-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Predicate | Subject |
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isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP22629 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419578768 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID161213 |
Total number of triples: 18.