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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008
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filingDate 2016-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106556692-B
titleOfInvention A method of influence being secreted on cell activity oxygen for detecting electronics tobacco product
abstract The present invention discloses a kind of method for secreting influence on cell activity oxygen for detecting electronics tobacco product comprising following steps: (1) pre-treatment of tested material;(2) preparation of human lung cancer cell A549 single cell suspension;(3) calculating of the cell concentration of human lung cancer cell A549 single cell suspension;(4) cell inoculation of human lung cancer cell A549 single cell suspension;(5) grouping of tested material;(6) setting of tested material detection dosage;(7) preparation of tested material culture product;(8) incubation of tested material;(9) positive control is added;(10) probe is loaded;(11) sample measures;(12) reactive oxygen species calculate.The present invention enables this method accurately and effectively to detect the method that electronics tobacco product influences cell activity oxygen level the optimal setting for being effectively treated, detecting dosage of sample and the correct selection of target cell.
priorityDate 2016-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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