http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106544360-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-50 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
filingDate | 2016-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106544360-B |
titleOfInvention | A method of terminating the transcription of lncRNA diallele |
abstract | The present invention provides a kind of methods of termination lncRNA diallele transcription, comprising the following steps: 1) building is respectively containing there are two types of the CMV- antibiotic-screening gene-PolyA DNA fragmentations of different antibiotic-screening genes;2) gRNA is designed according to different target genes, constructs Cas9/gRNA expression vector;3) inserting step 1 in target gene) obtained in CMV- antibiotic-screening gene-PolyA DNA fragmentation, construct the homologous recombination vector containing target gene;4) import cell: by obtained in step 3) containing target gene pair of homologous recombinant vector and step 2) obtained in the Cas9/gRNA expression vector cotransfection cells containing target gene;5) it is screened at least 2 weeks using associated antibiotic used in step 1), obtains the polyclonal cells strain that lncRNA diallele is knocked;This method may be implemented to be quickly obtained the purpose of lncRNA diallele while the cell strain of knockout to reach while efficient fixed point insertion. |
priorityDate | 2016-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 729.