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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
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filingDate 2016-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106544322-B
titleOfInvention It is a kind of for studying the reporting system and its construction method of Kiss1 gene expression regulation
abstract The present invention relates to a kind of for studying the reporting system and its construction method of Kiss1 gene expression regulation, and the system is that red fluorescent protein stablizes expression, double fluorescence Kiss1 gene expression regulation reporting systems that green fluorescence is regulated and controled by Kiss1 expression regulation element.Construction method includes: building red fluorescent protein targeting vector Ai9-dtomato;Construct green fluorescent protein targeting system;The building for the Cas9-GT1-7-Kiss1-2A-EGFP cell that EGFP is controlled by Kiss1 expression regulation element;Insertion stablize expression red fluorescence background to get.The present invention is realized to research object to the quantitative evaluation of Kiss1 action effect.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110308123-A
priorityDate 2016-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 48.