http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106525793-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-96491 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 |
| filingDate | 2016-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106525793-B |
| titleOfInvention | A method of MMP-9 is detected using fluorescence zymogram engram technology |
| abstract | The invention discloses a kind of methods using fluorescence zymogram engram technology detection MMP-9, belong to technical field of biotechnology, include: to carry out incubation centrifugally operated 1) to serum sample to be detected by gel protein concentrate method, the enzyme concentration in serum sample to be detected is improved 5 times；2) serum sample to be detected fluorescence zymogram blotting will be used after concentration, various enzymes and albumen in sample to be tested are separated using molecular weight area by electrophoresis；3) for the protein delivery for being distinguished step 2) by capillary blotting to nitrocellulose film surface, degradation is solidificated in the substrate of nitrocellulose film surface in advance；4) after bottom is degraded, fluorophor is separated with quenching group, issues fluorescence under special excitation wavelength, and the enzymatic activity of MMP9 in serum sample to be detected is judged by fluorescence intensity.The present invention is first concentrated serum, improves detection sensitivity under the premise of retaining gelatin zymography advantage.Then fluorescence zymogram blotting is recycled, so that experimental result background is low, it is sensitiveer, and result can quantify. |
| priorityDate | 2016-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.