http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106498082-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate | 2016-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106498082-B |
| titleOfInvention | Method for constructing ovarian cancer susceptibility gene variation library |
| abstract | The invention relates to a method for constructing an ovarian cancer susceptibility gene variation library, comprising the following steps: A. Designing primer pairs capable of detecting these variation regions according to the variation regions of ovarian cancer-related genes; B. Calculating and obtaining the judgment parameters of each primer pair R, the primer pair whose value of the judgment parameter R is less than or equal to 1 is classified as the first group of primer combination solutions, and the primer pair with the value of the judgment parameter R greater than 1 is classified as the second group of primer combination solution; C. DNA extraction and purification of the test sample; D. performing initial PCR amplification on the purified sample using the first set of primer combinations and the second set of primer combinations respectively; E. performing adapter ligation on the target fragment to obtain a fragment with an adapter; F. Using the mixture of the first set of primer combinations and the second set of primer combinations to perform library PCR amplification to obtain a sequencing library. The library constructed by the ovarian cancer susceptibility gene variation library construction method of the present invention has high sequencing throughput, strong sensitivity and strong specificity, and can be used to detect low-frequency mutations of free DNA. |
| priorityDate | 2016-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 200.