http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106498040-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
filingDate | 2016-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106498040-B |
titleOfInvention | A kind of molecular labeling microballon and the unicellular sequencing approach of high throughput based on the molecular labeling microballon |
abstract | A kind of unicellular sequencing approach of high throughput the invention discloses molecular labeling microballon and based on the molecular labeling microballon.A kind of molecular labeling microballon for high-throughput unicellular sequencing includes the oligonucleotide chain as molecular labeling of microballon ontology and coupling, and the oligonucleotide chain includes:Universal primer sequence, primer binding zone domain when as PCR amplification;Poly T tails are combined with the poly A sequences of mRNA;Molecular label sequence identifies the mRNA of combination;Cell sequence label, the cell that mark mRNA is derived from.The molecule labelled series of molecular labeling microballon of the present invention are divided into 4 functional areas, respectively universal primer sequence, cell sequence label, molecular label sequence and poly T tails, the unicellular sequencing approach of high throughput based on the molecular labeling microballon can disposably obtain up to ten thousand single celled specific transcriptional group informations, and the whole series experimental cost is low, the time is short, flux is high, has good application value. |
priorityDate | 2016-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.