http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106497973-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2016-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106497973-B |
| titleOfInvention | A kind of mankind and other mammalian cells adhere to body expression vector, expression system, preparation method and application |
| abstract | The invention discloses a kind of mankind and other mammalian cells to adhere to body expression vector, expression system, preparation method and application, belongs to genetic engineering and gene therapy technology field.Insertion is just like segmentation beta-interferon MAR sequence (or with the homologous nucleotide sequence of 95% or more sequence) shown in SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 on the attachment body expression vector, compare the expression vector comprising overall length MAR sequence or 387bp MAR sequence, carrier energy is efficient, continues, steadily expresses external source target gene, carrier expression especially comprising E sections of MAR sequences is best, can be used for gene therapy.It in the carrier, is segmented at multiple cloning sites namely the downstream eGFP of MAR sequence insertion vector, on the one hand can overcome transgene silencing, on the other hand can improve the expression of destination protein and the validity of subsequent monoclonal cell strain screening. |
| priorityDate | 2016-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 295.