http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106497962-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-37 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-885 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 |
| filingDate | 2016-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106497962-B |
| titleOfInvention | A Trichoderma engineering bacterium overexpressing lae1 gene and its construction method and application |
| abstract | The invention relates to a Trichoderma engineering bacterium strain overexpressing lae1 gene and its construction method and application. The construction method comprises the following steps: (1) Genomic DNA is extracted from Trichoderma SMF2, amplified by PCR, and the ORF region and the terminator sequence of the lae1 gene are prepared; (2) After the ORF region and the terminator sequence of the lae1 gene are digested, Ligated with the same enzyme-cut vector to obtain a recombinant plasmid; (3) transforming the recombinant plasmid into competent cells to obtain an overexpression plasmid; (4) linearizing the overexpression plasmid through enzyme digestion, and then transforming protoplasts to obtain Trichoderma engineering bacteria overexpressing lae1 gene. The Trichoderma engineering bacteria overexpressing the lae1 gene constructed by the invention can significantly increase the yield of the Trichoderma peptaibols, and contribute to the application of the Trichoderma peptaibols in medicine and agriculture. |
| priorityDate | 2016-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.