http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106496034-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H17-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C67-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C67-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C67-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-08 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C67-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C69-732 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C67-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C67-56 |
| filingDate | 2016-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106496034-B |
| titleOfInvention | Method a kind of while that separating chlorogenic acid and rutin sophorin are extracted from tobacco leaf |
| abstract | The invention discloses a kind of methods for extracting separating chlorogenic acid and rutin sophorin from tobacco leaf simultaneously.The tobacco leaf powder sample 200g for weighing 60 meshes, is dissolved in the water with solid-liquid ratio 1:20, temperature 50 C, and 120min is extracted in stirring.It collects filtrate and removes oil-soluble impurities with petroleum ether extraction to get chlorogenic acid, rutin sophorin extracting solution.Extracting solution upper prop carries out the column chromatography for separation of chlorogenic acid and rutin sophorin, first uses the water elution of 5 times of extracting liquid volumes, up to sugar-free detects in eluent.After being eluted with water, the ethanol gradient elution that eluent is various concentration is changed.The ethanol eluate of 10-15% concentration is collected respectively and the ethanol eluate of 30-40% concentration obtains solution of chlorogenic acid and rutin sophorin solution.This method save raw materials and reagents, reduce development cost, reach good separating effect. |
| priorityDate | 2016-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.