http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106480076-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
| filingDate | 2016-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106480076-B |
| titleOfInvention | Method for protecting high-efficiency recombinant expression of restriction endonuclease by methylase |
| abstract | The invention relates to a method for efficiently recombining and expressing restriction enzyme by utilizing protection of methylase, which is characterized by comprising the following steps: the methylase is M.Sss I; the restriction enzyme D is selected from: AatII, AciI, AclI, AfeI, AgeI, AscI, AsiSI, AvaI, BceAI, BmgBI, BsaAI, BsaHI, BseEI, BsiWI, BsmBI, BspDI, BspEI, BsrFI, BssHII, BstBI, BstUI, BtgZI, ClaI, EagI, FauI, FseI, FspI, HaeII, HgaI, HhaI, HinP1I, HpaII, Hpy99I, HpyCH4IV, KasI, MluI, NaeI, NarI, NgoMIV, NotI, NruI, Nt.BstI, Nt.CviPII, PaeR7I, PluTI, PmlI, PvuI, RsrII, SacII, SagriI, SragiI, SsaxI, SragiI, SxaI, SxapiI, SxaII, SxapiI, Tsaxii, TsaxiI, Tsaxii, Tsa. The construction process of the recombinant engineering bacteria directly uses the broad-spectrum protective methylase M.Sss I, does not need to perform complicated methylase gene screening aiming at single restriction endonuclease, has wide application range and greatly simplifies the recombinant expression process. |
| priorityDate | 2016-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.