http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106467739-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
| filingDate | 2015-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106467739-B |
| titleOfInvention | The specificity fluorescent probe of catechol O-methyltransferase and its application |
| abstract | A kind of specificity fluorescent probe of catechol O-methyltransferase and its application.The Specific probe is 3- benzothiazole -7,8- dihydroxycoumarin (3-BTD), the substrate can be catalyzed by COMT enzyme specificity and generate 8- methoxy based products and show fluorescence signal at 520nm, and the activity of COMT can be quantitative determined by the variation of fluorescence intensity.The probe cannot be only used for the qualitative assessment of COMT enzyme activity in separate sources biological sample, it may also be used for the inhibitor of external quickly screening COMT simultaneously assesses its rejection ability.This method can be used for the qualitative assessment of the true vigor of COMT enzyme in all kinds of external biological samples, realize the quick screening and the quantitative assessment of rejection ability of inhibitor；It can be used for evaluating the catalytic activity of different genera COMT and the COMT mutant with different aminoacids sequence, and then assess its ability for being metabolized pyrocatechol drug.With high sensitivity, accuracy is high, environment resistant interference performance is strong, is easy to the advantages such as high-throughput detection and inhibitor screening. |
| priorityDate | 2015-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.