| abstract |
The invention discloses a method for simultaneously detecting vitamin A and 25-hydroxyl vitamin D in blood. The method comprises the following steps: firstly, centrifuging a whole blood sample, and taking supernate to obtain serum or plasma for later use; secondly, calibrating a standard solution; thirdly, pretreating a sample; fourthly, taking 80mu L of to-be-detected supernate sample treated in the third step, putting the to-be-detected supernate sample into an automatic sample feeding bottle, and analyzing by liquid chromatography-tandem mass spectrometry; meanwhile, quantitatively detecting the contents of the vitamin A and the 25-hydroxyl vitamin D in the blood. The method for simultaneously detecting the vitamin A and the 25-hydroxyl vitamin D in the blood, disclosed by the invention, has the advantages of high specificity, high accuracy, high flexibility and short analysis time. |