http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106435024-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
filingDate | 2016-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106435024-B |
titleOfInvention | Fluorescent quantitative PCR primer, probe, kit and detection method for detecting avian influenza virus subtype |
abstract | The invention provides multiple fluorescent quantitative PCR primers and probes for detecting H5, H7 and H9 subtype influenza viruses, wherein the primer combined probe can simultaneously detect which subtype H5, H7 and H9 subtype influenza viruses are by adopting multiple fluorescent quantitative PCR, the primers of different subtypes have no mutual influence, the specificity is strong, the detection sensitivity is 10-50 copy numbers, the primers can accurately quantitatively and qualitatively detect a target sequence, the repeatability is good, and the reliability is high. The multiplex fluorescence quantitative PCR kit for simultaneously detecting H5, H7 and H9 subtype influenza viruses, provided by the invention, has the advantages of detection sensitivity of 10-50 copy numbers, high sensitivity and strong specificity. The application also provides that the detection method has good stability; the plasmid with gene fusion is used as the positive standard substance, so that the complicated operation of replacing the positive standard substance for multiple times is avoided, the detection time and the detection times are greatly shortened, and the sample can be detected within 2 hours. |
priorityDate | 2016-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 243.