http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106434743-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2016-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106434743-B |
| titleOfInvention | The method and its special culture media of transgenic wheat are obtained using electric shock introductory technique |
| abstract | The invention discloses a kind of methods and its special culture media that transgenic wheat is obtained using electric shock introductory technique, the immature embryo for selecting 1.2-1.5mm size is explant, it is incubated in the mannitol solution of 0.4M jointly 4-5 hours with 50-70 μ g/mL plasmid, pulse electric shock is carried out using the capacitor of the voltage of 500V/cm, 1500 μ F, select bar as riddled basins, rfp screens resistant plant as fluorescent reporter gene, with the glufosinate-ammonium of 5mg/L.Average conversion efficiency of the present invention is 1.0%, and red fluorescent protein expression rate is more than 90%.Its transformation period is short, stability is good, repetitive rate is high.A capable means are provided for the developing of genome editing technique practicability, an effective way is provided for genetic improvement of wheat industrialized development, has broad application prospects. |
| priorityDate | 2016-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.