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classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P33-02
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filingDate 2016-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106434727-B
titleOfInvention Construction method and application of simple arthrobacter engineering strain with high organic solvent tolerance
abstract The invention relates to construction and application of a simple arthrobacter engineering strain with high organic solvent tolerance. The invention obtains the engineering strain of the simple arthrobacter with high organic solvent tolerance by introducing gene segments into the simple arthrobacter; experiments prove that the tolerance of the engineering strain obtained by the construction method provided by the invention to methanol and ethanol is obviously improved. Steroid C is carried out by utilizing the engineering strain with high organic solvent tolerance obtained by the invention 1,2 The addition amount of the organic solvent in the system is improved by 1 time, the feeding concentration of the substrate is improved by 3 times, and the generation amount of the product is improved to different degrees; the construction and the application of the high organic solvent tolerance transformation engineering strain can improve the addition of the organic solvent in a transformation system, further improve the feeding concentration of the substrate and the yield of the product, and have important application value for improving the transformation efficiency of the high-concentration substrate of the steroid hydrophobic compounds.
priorityDate 2016-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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