http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106434545-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0665 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
filingDate | 2016-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106434545-B |
titleOfInvention | The separation method and kit of high-purity cord blood stem cell |
abstract | The present invention relates to the separation method of cord blood stem cell and kits.Wherein the method includes dilution step, settling step, stratification step and purification steps.Wherein, cell diluent in dilution step is PBS liquid or 0.8-1.0 weight % sodium-chloride water solution, the aqueous hetastarch solution that cell precipitation agent in settling step is 4-10 weight %, cell layering liquid in stratification step is the aqueous solution for being 1.074-1.076g/ml by the density that ficoll and/or cardiografin are configured to, and the cell purification liquid in purification step is the aqueous solution for being 1.090-1.095g/ml by the density that ficoll is configured to.Effective stem cell with higher purity can be obtained through the invention, and contaminating cell is less, is of great significance in clinical application. |
priorityDate | 2016-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.