http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106432440-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56911 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-23 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2016-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106432440-B |
| titleOfInvention | PPA-ELISA (polymerase chain reaction-enzyme linked immunosorbent assay) detection kit for Brucella melitensis antibody and preparation method of PPA-ELISA detection kit |
| abstract | The invention relates to a PPA-ELISA detection kit for brucellosis of sheep antibody, which is assembled by Pet-28a as a carrier, BL21 engineering bacteria as expression bacteria, IPTG induced expression of outer membrane proteins OMP25 and OMP31 of brucella suis S2 strain as coating antigens, a second antibody of rabbit anti-sheep IgG marked by horse radish peroxidase, an ELISA plate, confining liquid, washing buffer solution, enzyme-labeled second antibody diluent, sample diluent, positive control serum, negative control serum, TMB developing liquid and stop solution, and has the following beneficial effects: the kit takes Pet-28a as a carrier, and outer membrane proteins OMP25 and OMP31 of the Brucella melitensis S2 strain which are induced and expressed by IPTG are envelope antigens, so that the kit has good specificity, strong sensitivity and good repeatability to Brucella melitensis antigens through reasonable process steps, and can realize simultaneous detection of a large number of samples. |
| priorityDate | 2016-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.