http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106417251-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0221 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
| filingDate | 2016-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106417251-B |
| titleOfInvention | High-concentration vitrification solution and ultra-low temperature cryopreservation method for grouper embryos |
| abstract | The invention disclosesA high-concentration vitrification liquid and a method for freezing and preserving grouper embryos are disclosed, wherein the vitrification liquid comprises 60% of basic diluent, 35% of permeable antifreeze and 5% of non-permeable antifreeze; wherein the base diluent comprises NaCl 2 4.72 g/L，CaCl 2 ·2H 2 O 1.46 g/L，KCl 0.865 g/L，MgCl 2 6H2O 4.86.4 g/L and NaHCO 3 0.19g/L, dissolved in distilled water; the osmotic antifreeze agent comprises 15.75% of 1, 2-propylene glycol, 10.5% of methanol and 8.75% of glycerol; the non-osmotic antifreeze agent is sucrose or trehalose. The method comprises the following steps: preparing high-concentration permeable and non-permeable vitrification liquid, preparing eluent, screening frozen embryo, balancing embryo permeation in five steps, fast freezing and storing embryo, thawing embryo, eluting and culturing embryo. |
| priorityDate | 2016-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.