http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106399584-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate | 2016-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106399584-B |
| titleOfInvention | A kind of mandarin fish infectious spleen and kidney necrosis virus inactivation quick testing reagent kit and detection method |
| abstract | The invention discloses a kind of mandarin fish infectious spleen and kidney necrosis virus inactivation quick detection kit and detection methods.The detection kit and detection method are to monitor whether ISKNV inactivates by the detection to viral mRNA, comprising the concrete steps that will be 7 days after inactivated vaccine semifinished or finished goods inoculation CPB cell, extract cell total rna, remove DNA residual, carry out reverse transcription, then quantitative fluorescent PCR reaction is carried out with for mandarin fish infectious spleen and kidney necrosis virus ORF099 gene conserved region sequence design special primer and probe, determines whether virus inactivates completely according to reaction result.The present invention can inactivate mandarin fish infectious spleen and kidney necrosis virus and be used for quickly detecting, and can simplify ISKNV cell inactivation vaccine inactivation checked operation program, shorten round of visits, save the cost, improve inactivation and examine sensitivity, improve production of vaccine efficiency. |
| priorityDate | 2016-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.