http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106399371-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-03 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106399371-B |
| titleOfInvention | A method of building gene substitution mice study wild type and the non-flesh myoglobulin heavy chain II function of mutant |
| abstract | The present invention provides a kind of methods for constructing gene substitution mice study wild type and the non-flesh myoglobulin heavy chain II function of mutant.For the mpNTKV-LoxP carrier that the present invention is transformed more suitable for building gene targeting carrier especially gene substitution purpose, Pac I and the Swa I being newly added are the restriction enzymes for identifying eight bases, are conducive to the insertion of larger dna segment.MYH9 gene substitution (i.e. endogenous MYH9 gene is substituted by the non-flesh myoglobulin heavy chain II gene of the outer source code) targeting vector of building is transduceed by electricity in ES cells, by drug screening, ES cell monoclonal is selected, Southern Blot or PCR identification, the positive monoclonal cell rate that homologous recombination occurs is up to 90% or more, is easily obtained required cell line.MYH9 gene substitution allophenic mice is obtained using positive ES cells microinjection to Mouse Blastocysts, and the allophenic mice can mate passage, to obtain MYH9 gene substitution heterozygote and homozygote offspring. |
| priorityDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.